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Reconstruction of the microalga Nannochloropsis salina genome-scale metabolic model with applications to lipid production.


ABSTRACT: Nannochloropsis salina (= Eustigmatophyceae) is a marine microalga which has become a biotechnological target because of its high capacity to produce polyunsaturated fatty acids and triacylglycerols. It has been used as a source of biofuel, pigments and food supplements, like Omega 3. Only some Nannochloropsis species have been sequenced, but none of them benefit from a genome-scale metabolic model (GSMM), able to predict its metabolic capabilities.We present iNS934, the first GSMM for N. salina, including 2345 reactions, 934 genes and an exhaustive description of lipid and nitrogen metabolism. iNS934 has a 90% of accuracy when making simple growth/no-growth predictions and has a 15% error rate in predicting growth rates in different experimental conditions. Moreover, iNS934 allowed us to propose 82 different knockout strategies for strain optimization of triacylglycerols.iNS934 provides a powerful tool for metabolic improvement, allowing predictions and simulations of N. salina metabolism under different media and genetic conditions. It also provides a systemic view of N. salina metabolism, potentially guiding research and providing context to -omics data.

SUBMITTER: Loira N 

PROVIDER: S-EPMC5496344 | biostudies-literature | 2017 Jul

REPOSITORIES: biostudies-literature

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Reconstruction of the microalga Nannochloropsis salina genome-scale metabolic model with applications to lipid production.

Loira Nicolás N   Mendoza Sebastian S   Paz Cortés María M   Rojas Natalia N   Travisany Dante D   Genova Alex Di AD   Gajardo Natalia N   Ehrenfeld Nicole N   Maass Alejandro A  

BMC systems biology 20170704 1


<h4>Background</h4>Nannochloropsis salina (= Eustigmatophyceae) is a marine microalga which has become a biotechnological target because of its high capacity to produce polyunsaturated fatty acids and triacylglycerols. It has been used as a source of biofuel, pigments and food supplements, like Omega 3. Only some Nannochloropsis species have been sequenced, but none of them benefit from a genome-scale metabolic model (GSMM), able to predict its metabolic capabilities.<h4>Results</h4>We present i  ...[more]

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