How to speed up the polymerase chain reaction.
Ontology highlight
ABSTRACT:
SUBMITTER: Bustin SA
PROVIDER: S-EPMC5496742 | biostudies-literature | 2017 Jun
REPOSITORIES: biostudies-literature
Publications
How to speed up the polymerase chain reaction.
Biomolecular detection and quantification 20170620
Reducing the time taken to run qPCR assays on today's qPCR cyclers is rather straightforward and requires no specialised reagents or instruments. As the first article in a new series of short technical reports, I demonstrate that it is possible to reduce significantly both denaturation temperatures and cycling times, whilst retaining sensitivity and specificity of the original qPCR conditions. ...[more]
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