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Anaplasma phagocytophilum MSP4 and HSP70 Proteins Are Involved in Interactions with Host Cells during Pathogen Infection.


ABSTRACT: Anaplasma phagocytophilum transmembrane and surface proteins play a role during infection and multiplication in host neutrophils and tick vector cells. Recently, A. phagocytophilum Major surface protein 4 (MSP4) and Heat shock protein 70 (HSP70) were shown to be localized on the bacterial membrane, with a possible role during pathogen infection in ticks. In this study, we hypothesized that A. phagocytophilum MSP4 and HSP70 have similar functions in tick-pathogen and host-pathogen interactions. To address this hypothesis, herein we characterized the role of these bacterial proteins in interaction and infection of vertebrate host cells. The results showed that A. phagocytophilum MSP4 and HSP70 are involved in host-pathogen interactions, with a role for HSP70 during pathogen infection. The analysis of the potential protective capacity of MSP4 and MSP4-HSP70 antigens in immunized sheep showed that MSP4-HSP70 was only partially protective against pathogen infection. This limited protection may be associated with several factors, including the recognition of non-protective epitopes by IgG in immunized lambs. Nevertheless, these antigens may be combined with other candidate protective antigens for the development of vaccines for the control of human and animal granulocytic anaplasmosis. Focusing on the characterization of host protective immune mechanisms and protein-protein interactions at the host-pathogen interface may lead to the discovery and design of new effective protective antigens.

SUBMITTER: Contreras M 

PROVIDER: S-EPMC5496961 | biostudies-literature | 2017

REPOSITORIES: biostudies-literature

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<i>Anaplasma phagocytophilum</i> MSP4 and HSP70 Proteins Are Involved in Interactions with Host Cells during Pathogen Infection.

Contreras Marinela M   Alberdi Pilar P   Mateos-Hernández Lourdes L   Fernández de Mera Isabel G IG   García-Pérez Ana L AL   Vancová Marie M   Villar Margarita M   Ayllón Nieves N   Cabezas-Cruz Alejandro A   Valdés James J JJ   Stuen Snorre S   Gortazar Christian C   de la Fuente José J  

Frontiers in cellular and infection microbiology 20170705


<i>Anaplasma phagocytophilum</i> transmembrane and surface proteins play a role during infection and multiplication in host neutrophils and tick vector cells. Recently, <i>A. phagocytophilum</i> Major surface protein 4 (MSP4) and Heat shock protein 70 (HSP70) were shown to be localized on the bacterial membrane, with a possible role during pathogen infection in ticks. In this study, we hypothesized that <i>A. phagocytophilum</i> MSP4 and HSP70 have similar functions in tick-pathogen and host-pat  ...[more]

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