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Drosophila simulans: A Species with Improved Resolution in Evolve and Resequence Studies.

ABSTRACT: The combination of experimental evolution with high-throughput sequencing of pooled individuals-i.e., evolve and resequence (E&R)-is a powerful approach to study adaptation from standing genetic variation under controlled, replicated conditions. Nevertheless, E&R studies in Drosophila melanogaster have frequently resulted in inordinate numbers of candidate SNPs, particularly for complex traits. Here, we contrast the genomic signature of adaptation following ?60 generations in a novel hot environment for D. melanogaster and D. simulans For D. simulans, the regions carrying putatively selected loci were far more distinct, and thus harbored fewer false positives, than those in D. melanogaster We propose that species without segregating inversions and higher recombination rates, such as D. simulans, are better suited for E&R studies that aim to characterize the genetic variants underlying the adaptive response.

SUBMITTER: Barghi N

PROVIDER: S-EPMC5499140 | biostudies-literature | 2017 Jul

REPOSITORIES: biostudies-literature

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Drosophila simulans: A Species with Improved Resolution in Evolve and Resequence Studies.

Barghi Neda N Tobler Raymond R Nolte Viola V Schlötterer Christian C

G3 (Bethesda, Md.) 20170705 7

The combination of experimental evolution with high-throughput sequencing of pooled individuals-i.e., evolve and resequence (E&R)-is a powerful approach to study adaptation from standing genetic variation under controlled, replicated conditions. Nevertheless, E&R studies in Drosophila melanogaster have frequently resulted in inordinate numbers of candidate SNPs, particularly for complex traits. Here, we contrast the genomic signature of adaptation following ∼60 generations in a nov ...[more]

PMID: 28546383

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Evolve and Resequence studies in Drosophila simulans

Project description:Drosophila simulans: a species with improved resolution in Evolve and Resequence studies

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Meta-analysis suggests the microbiome responds to Evolve and Resequence experiments in Drosophila melanogaster.

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Optimizing the Power to Identify the Genetic Basis of Complex Traits with Evolve and Resequence Studies.

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