Ontology highlight
ABSTRACT:
SUBMITTER: A Rizvi SM
PROVIDER: S-EPMC5499539 | biostudies-literature | 2017 Jul
REPOSITORIES: biostudies-literature
A Rizvi Syed M SM Prajapati Hemant K HK Nag Purba P Ghosh Santanu K SK
Nucleic acids research 20170701 12
The 2-μm plasmid of the budding yeast Saccharomyces cerevisiae achieves a high chromosome-like stability with the help of four plasmid-encoded (Rep1, Rep2, Raf1 and Flp) and several host-encoded proteins. Rep1 and Rep2 and the DNA locus STB form the partitioning system ensuring equal segregation of the plasmid. The Flp recombinase and its target sites FRTs form the amplification system which is responsible for the steady state plasmid copy number. In this work we show that the absence of Raf1 ca ...[more]