Ontology highlight
ABSTRACT:
SUBMITTER: Lopez-Alonso JP
PROVIDER: S-EPMC5499641 | biostudies-literature | 2017 Jun
REPOSITORIES: biostudies-literature
López-Alonso Jorge Pedro JP Kaminishi Tatsuya T Kikuchi Takeshi T Hirata Yuya Y Iturrioz Idoia I Dhimole Neha N Schedlbauer Andreas A Hase Yoichi Y Goto Simon S Kurita Daisuke D Muto Akira A Zhou Shu S Naoe Chieko C Mills Deryck J DJ Gil-Carton David D Takemoto Chie C Himeno Hyouta H Fucini Paola P Connell Sean R SR
Nucleic acids research 20170601 11
During 30S ribosomal subunit biogenesis, assembly factors are believed to prevent accumulation of misfolded intermediate states of low free energy that slowly convert into mature 30S subunits, namely, kinetically trapped particles. Among the assembly factors, the circularly permuted GTPase, RsgA, plays a crucial role in the maturation of the 30S decoding center. Here, directed hydroxyl radical probing and single particle cryo-EM are employed to elucidate RsgA΄s mechanism of action. Our results s ...[more]