Genome-wide retinal transcriptome analysis of endotoxin-induced uveitis in mice with next-generation sequencing.

ABSTRACT:

Purpose

Endotoxin-induced uveitis (EIU) is a well-established mouse model for studying human acute inflammatory uveitis. The purpose of this study is to investigate the genome-wide retinal transcriptome profile of EIU.

Methods

The anterior segment of the mice was examined with a slit-lamp, and clinical scores were evaluated simultaneously. The histological changes in the posterior segment of the eyes were evaluated with hematoxylin and eosin (H&E) staining. A high throughput RNA sequencing (RNA-seq) strategy using the Illumina Hiseq 2500 platform was applied to characterize the retinal transcriptome profile from lipopolysaccharide (LPS)-treated and untreated mice. The validation of the differentially expressed genes (DEGs) was analyzed with real-time PCR.

Results

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SUBMITTER: Qiu Y 

PROVIDER: S-EPMC5501689 | biostudies-literature | 2017

REPOSITORIES: biostudies-literature