DNA-polyfluorophore excimers as sensitive reporters for esterases and lipases.
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ABSTRACT:
SUBMITTER: Dai N
PROVIDER: S-EPMC5501962 | biostudies-literature | 2010 Feb
REPOSITORIES: biostudies-literature
Publications
DNA-polyfluorophore excimers as sensitive reporters for esterases and lipases.
Dai Nan N Teo Yin Nah YN Kool Eric T ET
Chemical communications (Cambridge, England) 20100122 8
DNA-scaffolded oligodeoxyriboside fluorophores (ODFs) were used as the reporters in turn-on sensing of enzymatic bond-cleaving activity. A tetramer ODF of pyrene deoxynucleosides displayed high quenching efficiency when conjugated via ester linkages with a dabcyl quencher, and yielded large signal increases with several enzymes in vitro and in intact human cells. ...[more]
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