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Variation of active constituents and antioxidant activity in Scabiosa tschiliensis Grunning from different stages.

ABSTRACT: It has been claimed that inflorescences of Scabiosa tschiliensis Grunning (ST) may prevent liver diseases because of its higher chlorogenic acid. There was scant information on the phytochemical profiles and antioxidant activities of the whole plant from different growing stages. The changes of active-compounds and antioxidant activities of ST from three growing stages were studied. Total phenolic and flavonoid contents were analyzed and ranged from 0.00 to 140.03 mg GAE/g and 9.10 to 460.01 mg RE/g, respectively. The pre-flowering stage ethyl acetate (PFSEA) fraction of ST appeared to contain the highest content of chlorogenic acid, and demonstrated the highest DPPH radical-scavenging activity with the IC₅₀ value of 8.47 ± 0.23 µg/mL which was nearly equal to the IC₅₀ value of vitamin C (7.60 ± 0.61 µg/mL). Principal component analysis suggested that the PFSEA fraction of ST might be a desirable antioxidant natural resource due to the highest potential antioxidant properties.

SUBMITTER: Wang J

PROVIDER: S-EPMC5502020 | biostudies-literature | 2017 Jul

REPOSITORIES: biostudies-literature

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Variation of active constituents and antioxidant activity in <i>Scabiosa tschiliensis</i> Grunning from different stages.

Wang Junli J Liu Kun K Li Xiaoxu X Bi Kaili K Zhang Yiming Y Huang Jingjing J Zhang Rongrong R

Journal of food science and technology 20170612 8

It has been claimed that inflorescences of <i>Scabiosa tschiliensis</i> Grunning (ST) may prevent liver diseases because of its higher chlorogenic acid. There was scant information on the phytochemical profiles and antioxidant activities of the whole plant from different growing stages. The changes of active-compounds and antioxidant activities of ST from three growing stages were studied. Total phenolic and flavonoid contents were analyzed and ranged from 0.00 to 140.03 mg GAE/g and 9.10 to 460 ...[more]

PMID: 28740285

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Project description:BackgroundCosmos caudatus is an annual plant known for its medicinal value in treating several health conditions, such as high blood pressure, arthritis, and diabetes mellitus. The α-glucosidase inhibitory activity and total phenolic content of the leaf aqueous ethanolic extracts of the plant at different growth stages (6, 8. 10, 12 and 14 weeks) were determined in an effort to ascertain the best time to harvest the plant for maximum medicinal quality with respect to its glucose-lowering effects.MethodsThe aqueous ethanolic leaf extracts of C. caudatus were characterized by NMR and LC-MS/MS. The total phenolic content and α-glucosidase inhibitory activity were evaluated by the Folin-Ciocalteu method and α-glucosidase inhibitory assay, respectively. The statistical significance of the results was evaluated using one-way ANOVA with Duncan's post hoc test, and correlation among the different activities was performed by Pearson's correlation test. NMR spectroscopy along with multivariate data analysis was used to identify the metabolites correlated with total phenolic content and α-glucosidase inhibitory activity of the C. caudatus leaf extracts.ResultsIt was found that the α-glucosidase inhibitory activity and total phenolic content of the optimized ethanol:water (80:20) leaf extract of the plant increased significantly as the plant matured, reaching a maximum at the 10th week. The IC50 value for α-glucosidase inhibitory activity (39.18 μg mL- 1) at the 10th week showed greater potency than the positive standard, quercetin (110.50 μg mL- 1). Through an 1H NMR-based metabolomics approach, the 10-week-old samples were shown to be correlated with a high total phenolic content and α-glucosidase inhibitory activity. From the partial least squares biplot, rutin and flavonoid glycosides, consisting of quercetin 3-O-arabinofuranoside, quercetin 3-O-rhamnoside, quercetin 3-O-glucoside, and quercetin 3-O-xyloside, were identified as the major bioactive metabolites. The metabolites were identified by NMR spectroscopy (J-resolve, HSQC and HMBC experiments) and further supported by dereplication via LC-MS/MS.ConclusionFor high phytomedicinal quality, the 10th week is recommended as the best time to harvest C. caudatus leaves with respect to its glucose lowering potential.

Dataset Information

Variation of active constituents and antioxidant activity in Scabiosa tschiliensis Grunning from different stages.

Publications

Variation of active constituents and antioxidant activity in <i>Scabiosa tschiliensis</i> Grunning from different stages.

Similar Datasets

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