Ontology highlight
ABSTRACT:
SUBMITTER: Kumari V
PROVIDER: S-EPMC5502754 | biostudies-literature | 2017 May
REPOSITORIES: biostudies-literature
Journal of cystic fibrosis : official journal of the European Cystic Fibrosis Society 20170421 3
<h4>Background</h4>AP2 is a clathrin-based endocytic adaptor complex comprising α, β2, μ2 and σ2 subunits. μ2 regulates CFTR endocytosis. The α subunit interacts with CFTR in the intestine but its physiologic significance is unclear.<h4>Methods</h4>CFTR short circuit current was measured in intestinal T84 cells following shRNA knock down of AP2α (AP2αKD). Clathrin-coated structures (CCS) were immunolabeled and quantified in AP2αKD intestinal Caco2BBe (C2BBe) cells. GST tagged human AP2α appendag ...[more]