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AhpC of the mycobacterial antioxidant defense system and its interaction with its reducing partner Thioredoxin-C.


ABSTRACT: Despite the highly oxidative environment of the phagosomal lumen, the need for maintaining redox homeostasis is a critical aspect of mycobacterial biology. The pathogens are equipped with the sophisticated thioredoxin- (Trx) and peroxiredoxin system, including TrxC and the alkyl hydroperoxide reductase subunit C (AhpC), whereby TrxC is one of the reducing partners of AhpC. Here we visualize the redox modulated dodecamer ring formation of AhpC from Mycobacterium bovis (BCG strain; MbAhpC) using electron microscopy and present novel insights into the unique N-terminal epitope (40 residues) of mycobacterial AhpC. Truncations and amino acid substitutions of residues in the unique N-terminus of MbAhpC provide insights into their structural and enzymatic roles, and into the evolutionary divergence of mycobacterial AhpC versus that of other bacteria. These structural details shed light on the epitopes and residues of TrxC which contributes to its interaction with AhpC. Since human cells lack AhpC, the unique N-terminal epitope of mycobacterial AhpC as well as the MbAhpC-TrxC interface represent an ideal drug target.

SUBMITTER: Wong CF 

PROVIDER: S-EPMC5505994 | biostudies-literature | 2017 Jul

REPOSITORIES: biostudies-literature

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AhpC of the mycobacterial antioxidant defense system and its interaction with its reducing partner Thioredoxin-C.

Wong Chui Fann CF   Shin Joon J   Subramanian Manimekalai Malathy Sony MS   Saw Wuan Geok WG   Yin Zhan Z   Bhushan Shashi S   Kumar Arvind A   Ragunathan Priya P   Grüber Gerhard G  

Scientific reports 20170711 1


Despite the highly oxidative environment of the phagosomal lumen, the need for maintaining redox homeostasis is a critical aspect of mycobacterial biology. The pathogens are equipped with the sophisticated thioredoxin- (Trx) and peroxiredoxin system, including TrxC and the alkyl hydroperoxide reductase subunit C (AhpC), whereby TrxC is one of the reducing partners of AhpC. Here we visualize the redox modulated dodecamer ring formation of AhpC from Mycobacterium bovis (BCG strain; MbAhpC) using e  ...[more]

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