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Insulin-binding aptamer-conjugated graphene oxide for insulin detection.

ABSTRACT: This paper describes a simple and sensitive aptamer/graphene oxide (GO) based assay for insulin detection. GO can protect DNA from nuclease cleavage, but aptamers can be detached from the GO surface by specific target binding. This exposes the aptamers to enzymatic cleavage and releases the target for a new cycle. Cycling of targets leads to significant signal amplification and low LOD.

SUBMITTER: Pu Y

PROVIDER: S-EPMC5508966 | biostudies-literature | 2011 Oct

REPOSITORIES: biostudies-literature

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Insulin-binding aptamer-conjugated graphene oxide for insulin detection.

Pu Ying Y Zhu Zhi Z Han Da D Liu Huixia H Liu Jun J Liao Jie J Zhang Kejing K Tan Weihong W

The Analyst 20110826 20

This paper describes a simple and sensitive aptamer/graphene oxide (GO) based assay for insulin detection. GO can protect DNA from nuclease cleavage, but aptamers can be detached from the GO surface by specific target binding. This exposes the aptamers to enzymatic cleavage and releases the target for a new cycle. Cycling of targets leads to significant signal amplification and low LOD. ...[more]

PMID: 21874167

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