Impact of environmental microbiota on human microbiota of workers in academic mouse research facilities: An observational study.

ABSTRACT:

Objectives

To characterize the microbial environment of workers in academic mouse research facilities using endotoxin, 16S qPCR, and 16S amplicon sequencing. To determine whether the work microbiome contributes to the human microbiome of workers.

Methods

We performed area air sampling from the animal rooms, dirty, middle, and setup cage wash locations in four academic mouse research facilities. 10 workers in the dirty cage wash area underwent personal air sampling as well as repeated collection of nasal, oral, and skin samples before and after the work shift. Environmental samples underwent measurement of endotoxin, mouse allergen, bacteria copy number via 16S qPCR, and microbial identification via 16S rDNA sequencing. 16S rDNA sequencing was also performed on human samples

SUBMITTER: Lai PS 

PROVIDER: S-EPMC5509249 | biostudies-literature | 2017

REPOSITORIES: biostudies-literature