Microfluidic assay of circulating endothelial cells in coronary artery disease patients with angina pectoris.

ABSTRACT:

Background

Circulating endothelial cells (CECs) are widely reported as a promising biomarker of endothelial damage/dysfunction in coronary artery disease (CAD). The two popular methods of CEC quantification include the use of immunomagnetic beads separation (IB) and flow cytometry analysis (FC); however, they suffer from two main shortcomings that affect their diagnostic and prognostic responses: non-specific bindings of magnetic beads to non-target cells and a high degree of variability in rare cell identification, respectively. We designed a microfluidic chip with spatially staggered micropillars for the efficient harvesting of CECs with intact cellular morphology in an attempt to revisit the diagnostic goal of CEC counts in CAD patients with angina pectoris.

Methods

A lab

SUBMITTER: Chen S 

PROVIDER: S-EPMC5509377 | biostudies-literature | 2017

REPOSITORIES: biostudies-literature