Ontology highlight
ABSTRACT:
SUBMITTER: Schuck BW
PROVIDER: S-EPMC5510169 | biostudies-literature | 2017 Apr
REPOSITORIES: biostudies-literature
Schuck Brittany W BW MacArthur Ryan R Inglese James J
Current protocols in neuroscience 20170410
Reporter-biased artifacts-i.e., compounds that interact directly with the reporter enzyme used in a high-throughput screening (HTS) assay and not the biological process or pharmacology being interrogated-are now widely recognized to reduce the efficiency and quality of HTS used for chemical probe and therapeutic development. Furthermore, narrow or single-concentration HTS perpetuates false negatives during primary screening campaigns. Titration-based HTS, or quantitative HTS (qHTS), and coincide ...[more]