Manipulation of nuclear architecture through CRISPR-mediated chromosomal looping.
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ABSTRACT:
SUBMITTER: Morgan SL
PROVIDER: S-EPMC5511349 | biostudies-literature | 2017 Jul
REPOSITORIES: biostudies-literature
Publications
Manipulation of nuclear architecture through CRISPR-mediated chromosomal looping.
Morgan Stefanie L SL Mariano Natasha C NC Bermudez Abel A Arruda Nicole L NL Wu Fangting F Luo Yunhai Y Shankar Gautam G Jia Lin L Chen Huiling H Hu Ji-Fan JF Hoffman Andrew R AR Huang Chiao-Chain CC Pitteri Sharon J SJ Wang Kevin C KC
Nature communications 20170713
Chromatin looping is key to gene regulation, yet no broadly applicable methods to selectively modify chromatin loops have been described. We have engineered a method for chromatin loop reorganization using CRISPR-dCas9 (CLOuD9) to selectively and reversibly establish chromatin loops. We demonstrate the power of this technology to selectively modulate gene expression at targeted loci. ...[more]
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