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Metagenomic data of free cyanide and thiocyanate degrading bacterial communities.

ABSTRACT: The data presented in this article contains the bacterial community structure of the free cyanide (CN-) and thiocyanate (SCN-) degrading organisms that were isolated from electroplating wastewater and synthetic SCN- containing wastewater. PCR amplification of the 16S rRNA V1-V3 regions was undertaken using the 27F and 518R oligonucleotide primers following the metacommunity DNA extraction procedure. The PCR amplicons were processed using the illumina® reaction kits as per manufacturer?s instruction and sequenced using the illumina® MiSeq-2000, using the MiSeq V3 kit. The data was processed using bioinformatics tools such as QIIME and the raw sequence files are available via NCBI?s Sequence Read Archive (SRA) database.

SUBMITTER: Mekuto L

PROVIDER: S-EPMC5512189 | biostudies-literature | 2017 Aug

REPOSITORIES: biostudies-literature

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Metagenomic data of free cyanide and thiocyanate degrading bacterial communities.

Mekuto Lukhanyo L Ntwampe Seteno K O SKO Mudumbi John B N JBN Akinpelu Enoch A EA Mewa-Ngongang Maxwell M

Data in brief 20170704

The data presented in this article contains the bacterial community structure of the free cyanide (CN-) and thiocyanate (SCN-) degrading organisms that were isolated from electroplating wastewater and synthetic SCN- containing wastewater. PCR amplification of the 16S rRNA V1-V3 regions was undertaken using the 27F and 518R oligonucleotide primers following the metacommunity DNA extraction procedure. The PCR amplicons were processed using the illumina® reaction ki ...[more]

PMID: 28748209

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