Ontology highlight
ABSTRACT:
SUBMITTER: Liu Y
PROVIDER: S-EPMC5512609 | biostudies-literature | 2016 Jun
REPOSITORIES: biostudies-literature
Liu Yanfeng Y Link Hannes H Liu Long L Du Guocheng G Chen Jian J Sauer Uwe U
Nature communications 20160621
Recent advances in genome engineering have further widened the gap between our ability to implement essentially any genetic change and understanding the impact of these changes on cellular function. We lack efficient methods to diagnose limiting steps in engineered pathways. Here, we develop a generally applicable approach to reveal limiting steps within a synthetic pathway. It is based on monitoring metabolite dynamics and simplified kinetic modelling to differentiate between putative causes of ...[more]