Dataset Information

Transformation of patchouli alcohol to ?-patchoulene by gastric juice: ?-patchoulene is more effective in preventing ethanol-induced gastric injury.

ABSTRACT: Pogostemonis Herba is a functional food approved in Asian countries. Its major constituent, patchouli alcohol (PA), possesses a gastroprotective effect and is reported to transform into ?-patchoulene (?-PAE) under acidic conditions. To investigate whether ?-PAE, the metabolite of PA, has a protective effect on the gastrointestinal tract, the formation of ?-PAE by gastric juice and the anti-ulcerogenic potential of ?-PAE against ethanol-induced gastric injury were evaluated. The Results indicated that PA was converted to ?-PAE by rat gastric juice. Additionally, ?-PAE was significantly better than PA at reducing the area of gastric ulcer. The overproduction of malondialdehyde, tumour necrosis factor-?, interleukin (IL)-1?, IL-6, Fas, FasL and caspase-3 was markedly inhibited by ?-PAE while the underproduction of superoxide dismutase, glutathione and catalase was significantly improved. ?-PAE also regulated the NF-?B and ERK1/2 signalling pathways. Our findings suggest that ?-PAE has potential therapeutic efficacy for antiulcer treatment.

SUBMITTER: Liu Y

PROVIDER: S-EPMC5514077 | biostudies-literature | 2017 Jul

REPOSITORIES: biostudies-literature

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Transformation of patchouli alcohol to β-patchoulene by gastric juice: β-patchoulene is more effective in preventing ethanol-induced gastric injury.

Liu Yuhong Y Liang Jiali J Wu Jiazhen J Chen Hanbin H Zhang Zhenbiao Z Yang Hongmei H Chen Liping L Chen Haiming H Su Ziren Z Li Yucui Y

Scientific reports 20170717 1

Pogostemonis Herba is a functional food approved in Asian countries. Its major constituent, patchouli alcohol (PA), possesses a gastroprotective effect and is reported to transform into β-patchoulene (β-PAE) under acidic conditions. To investigate whether β-PAE, the metabolite of PA, has a protective effect on the gastrointestinal tract, the formation of β-PAE by gastric juice and the anti-ulcerogenic potential of β-PAE against ethanol-induced gastric injury were evaluated. The Results indicated ...[more]

PMID: 28717228

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Project description:BackgroundG protein-coupled bile acid receptor 1 (GPBAR1) is a G protein-coupled receptor for bile acids, which is widely expressed in many human tissues. Patchouli alcohol (PA) has been shown to have an anti-cancer effect, including in prostate cancer (PCa). This study sought to confirm the regulatory mechanism of GPBAR1 in the anti-cancer activity of PA in PCa.MethodsThe SwissTargetPrediction website (Pro >0) was used to predict the target of PA. The UALCAN and The Cancer Genome Atlas-Prostate cohort was used to examine the differentially expressed genes and PCa recurrence. A gene set enrichment analysis (GSEA) was conducted to analyze the relationship between the expression of GPBAR1 and PCa proliferation, migration, and invasion. Cell proliferation, migration, and invasion were assessed by colony formation, 5-Ethynyl-2'-deoxyuridine staining, cell scratch assays, and Transwell invasion assays, respectively. A xenograft animal model was established to assess the effect of PA on tumor growth in vivo. GPBAR1 protein and apoptosis related protein expression was measured by western blot.ResultsGPBAR1 was a PA target predicted by the SwissTargetPrediction website. PA inhibited the expression of GPBAR1 in PCa cells in a time- and dose-dependent manner. The abnormal expression of GPBAR1 was related to cell proliferation, migration, and invasion. Additionally, GPBAR1 overexpression promoted the cell proliferation, migration, and invasion, and inhibited the apoptosis of PCa cells. GPBAR1 silencing inhibited the cell proliferation, migration, and invasion, and promoted the apoptosis of PCa cells. High expressions of GPBAR1 suppressed tumor growth in tumor-bearing mice. Further, GPBAR1 promoted the activation of nuclear factor kappa B (NF-κB) signaling, and PA regulated the malignant phenotypes of PCa cells via the NF-κB signaling pathway mediated by GPBAR1.ConclusionsGPBAR1 is a promising drug target of PA, and was shown to regulate the proliferation, apoptosis, migration, and invasion of PCa cells through GPBAR1/NF-κB inhibition.

Dataset Information

Transformation of patchouli alcohol to ?-patchoulene by gastric juice: ?-patchoulene is more effective in preventing ethanol-induced gastric injury.

Publications

Transformation of patchouli alcohol to β-patchoulene by gastric juice: β-patchoulene is more effective in preventing ethanol-induced gastric injury.

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