Dataset Information

Fine mapping of qSKC-1, a major quantitative trait locus for shoot K⁺ concentration, in rice seedlings grown under salt stress.

ABSTRACT: Shoot K⁺ concentration (SKC) is an important physiological parameter used to evaluate salt tolerance at the seedling stage in rice (Oryza sativa L.). qSKC-1, a major quantitative trait locus for SKC in rice under salt stress, was detected on chromosome 1 using three F₂ populations constructed by crossing 'Nipponbare' and its two salt-sensitive mutants (rss2 and rss4) with an indica cultivar 'Zhaiyeqing8' ('ZYQ8'). In this study, the chromosomal location of qSKC-1 was determined precisely by fine mapping. First, the presence of qSKC-1 was confirmed by QTL analysis of a re-constructed 'Nipponbare' × 'ZYQ8' F₂ population. Then, F₂ plants in which recombination events had occurred near qSKC-1 were identified from the 'Nipponbare' × 'ZYQ8' and rss4 × 'ZYQ8' F₂ populations, and their phenotypic values were confirmed by progeny tests. Eventually, by analyzing recombination events in these recombinants, the qSKC-1 locus was mapped precisely to 445 kb between markers RM578 and IM8854. These results will facilitate map-based cloning of the qSKC-1 locus.

SUBMITTER: Jing W

PROVIDER: S-EPMC5515313 | biostudies-literature | 2017 Jun

REPOSITORIES: biostudies-literature

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Fine mapping of qSKC-1, a major quantitative trait locus for shoot K+ concentration, in rice seedlings grown under salt stress.

Jing Wen W Deng Ping P Cao Chengjuan C Zhang Wenhua W

Breeding science 20170530 3

Shoot K+ concentration (SKC) is an important physiological parameter used to evaluate salt tolerance at the seedling stage in rice (Oryza sativa L.). qSKC-1, a major quantitative trait locus for SKC in rice under salt stress, was detected on chromosome 1 using three F2 populations constructed by crossing 'Nipponbare' and its two salt-sensitive mutants (rss2 and rss4) with an indica cultivar 'Zhaiyeqing8' ('ZYQ8'). In this study, the chromo ...[more]

PMID: 28744182

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Dataset Information

Fine mapping of qSKC-1, a major quantitative trait locus for shoot K⁺ concentration, in rice seedlings grown under salt stress.

Publications

Fine mapping of <i>qSKC-1</i>, a major quantitative trait locus for shoot K<sup>+</sup> concentration, in rice seedlings grown under salt stress.

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