Identification of ciliated sensory neuron-expressed genes in Caenorhabditis elegans using targeted pull-down of poly(A) tails.
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ABSTRACT:
SUBMITTER: Kunitomo H
PROVIDER: S-EPMC551537 | biostudies-literature | 2005
REPOSITORIES: biostudies-literature
Publications
Identification of ciliated sensory neuron-expressed genes in Caenorhabditis elegans using targeted pull-down of poly(A) tails.
Kunitomo Hirofumi H Uesugi Hiroko H Kohara Yuji Y Iino Yuichi Y
Genome biology 20050131 2
It is not always easy to apply microarray technology to small numbers of cells because of the difficulty in selectively isolating mRNA from such cells. We report here the preparation of mRNA from ciliated sensory neurons of Caenorhabditis elegans using the mRNA-tagging method, in which poly(A) RNA was co-immunoprecipitated with an epitope-tagged poly(A)-binding protein specifically expressed in sensory neurons. Subsequent cDNA microarray analyses led to the identification of a panel of sensory n ...[more]
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