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A universal method for automated gene mapping.

ABSTRACT: Small insertions or deletions (InDels) constitute a ubiquituous class of sequence polymorphisms found in eukaryotic genomes. Here, we present an automated high-throughput genotyping method that relies on the detection of fragment-length polymorphisms (FLPs) caused by InDels. The protocol utilizes standard sequencers and genotyping software. We have established genome-wide FLP maps for both Caenorhabditis elegans and Drosophila melanogaster that facilitate genetic mapping with a minimum of manual input and at comparatively low cost.

SUBMITTER: Zipperlen P

PROVIDER: S-EPMC551539 | biostudies-literature | 2005

REPOSITORIES: biostudies-literature

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A universal method for automated gene mapping.

Zipperlen Peder P Nairz Knud K Rimann Ivo I Basler Konrad K Hafen Ernst E Hengartner Michael M Hajnal Alex A

Genome biology 20050117 2

Small insertions or deletions (InDels) constitute a ubiquituous class of sequence polymorphisms found in eukaryotic genomes. Here, we present an automated high-throughput genotyping method that relies on the detection of fragment-length polymorphisms (FLPs) caused by InDels. The protocol utilizes standard sequencers and genotyping software. We have established genome-wide FLP maps for both Caenorhabditis elegans and Drosophila melanogaster that facilitate genetic mapping with a minimum of manual ...[more]

PMID: 15693948

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