Ontology highlight
ABSTRACT:
SUBMITTER: Ryu YS
PROVIDER: S-EPMC5515457 | biostudies-literature | 2017
REPOSITORIES: biostudies-literature
Ryu Young Shin YS Chandran Sathesh-Prabu SP Kim Kyungchul K Lee Sung Kuk SK
PloS one 20170718 7
The ability to precisely and seamlessly modify a target genome is needed for metabolic engineering and synthetic biology techniques aimed at creating potent biosystems. Herein, we report on a promising method in Escherichia coli that relies on the insertion of an optimized tetA dual selection cassette followed by replacement of the same cassette with short, single-stranded DNA (oligos) or long, double-stranded DNA and the isolation of recombinant strains by negative selection using NiCl2. This m ...[more]