FISH-ing for captured contacts: towards reconciling FISH and 3C.
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ABSTRACT:
SUBMITTER: Fudenberg G
PROVIDER: S-EPMC5517086 | biostudies-literature | 2017 Jul
REPOSITORIES: biostudies-literature
Publications
FISH-ing for captured contacts: towards reconciling FISH and 3C.
Fudenberg Geoffrey G Imakaev Maxim M
Nature methods 20170612 7
Chromosome conformation capture (3C) and fluorescence in situ hybridization (FISH) are two widely used technologies that provide distinct readouts of 3D chromosome organization. While both technologies can assay locus-specific organization, how to integrate views from 3C, or genome-wide Hi-C, and FISH is far from solved. Contact frequency, measured by Hi-C, and spatial distance, measured by FISH, are often assumed to quantify the same phenomena and used interchangeably. Here, however, we demonst ...[more]
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