Dataset Information

Volatile-Mediated Attraction of Greenhouse Whitefly Trialeurodes vaporariorum to Tomato and Eggplant.

ABSTRACT: The behavior of the greenhouse whitefly, Trialeurodes vaporariorum Westwood (Hemiptera: Aleyrodidae), is known to be affected by plant volatile cues, but its attraction or repellent to specific volatile cues has not been deeply studied yet. Therefore, the aim of our study was to identify the most attractive plant among cultivars of tomato (Solanum lycopersicum) and eggplant (Solanum melongena) to evaluate the volatiles of plants to identify the chemical compound(s) that attract T. vaporariorum. We speculated that whitefly-host plant interaction primarily depends on plant volatile emissions and that once the plant is damaged, it might attract more whiteflies. Three intact (uninfested) tomato, four intact eggplant cultivars and whitefly infested plants of the most whitefly attractive tomato and eggplant cultivars were examined by behavioral assay experiments for attractiveness to T. vaporariorum and headspace volatile were determined by solid-phase microextraction (SPME) and gas chromatography-mass spectrometry. Whiteflies had the highest preference for the intact eggplant Kuai Yuan Qie (KYQ) among the eggplant and the tomato plant cultivars in bioassay experiments. Although both male and female whiteflies were significantly more attracted to infested KYQ plants than to intact plants, whitefly females did not select conspecific-infested YG plants. The volatile emissions among different plant cultivars in individual species and infested versus intact plants were significantly different. Among these volatiles, identified major green leaf volatiles [(Z)-3-hexen-1-ol] and terpenoids [?-pinene, (E)-?-caryophyllene, ?-humulene, azulene] showed a constitutive relationship with the most whitefly preference plants. Our findings provide new insights into the chemical compounds that attract or repel whiteflies.

SUBMITTER: Darshanee HLC

PROVIDER: S-EPMC5517405 | biostudies-literature | 2017

REPOSITORIES: biostudies-literature

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Volatile-Mediated Attraction of Greenhouse Whitefly Trialeurodes vaporariorum to Tomato and Eggplant.

Darshanee Hewa L C HLC Ren Hui H Ahmed Nazeer N Zhang Zhan-Feng ZF Liu Yan-Hong YH Liu Tong-Xian TX

Frontiers in plant science 20170720

The behavior of the greenhouse whitefly, Trialeurodes vaporariorum Westwood (Hemiptera: Aleyrodidae), is known to be affected by plant volatile cues, but its attraction or repellent to specific volatile cues has not been deeply studied yet. Therefore, the aim of our study was to identify the most attractive plant among cultivars of tomato (Solanum lycopersicum) and eggplant (Solanum melongena) to evaluate the volatiles of plants to identify the chemical compound(s) that attr ...[more]

PMID: 28775733

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Project description:The greenhouse whitefly Trialeurodes vaporariorum Westwood (Hemiptera: Aleyrodidae) is a serious pest of many fruits, vegetables and ornamental crops in sub-tropical regions and in greenhouses worldwide. Potato is a secondary host of the species but the major threat from this insect in the Andean region for potato is the transmission by this insect of a crinivirus causing Potato yellow vein disease. Determination of the pest's temperature-dependent population growth potential is crucial knowledge for understanding the population dynamics and spread potential of the species and the diseases it can transmit, as well as for designing effective pest management strategies. Trialeurodes vaporariorum development, mortality and reproduction were studied at seven constant temperatures ranging from 10 to 32 °C. The Insect Life Cycle Modeling (ILCYM) software was used to fit nonlinear equations to the data and establish an overall phenology model to simulate life-table parameters based on temperature. In addition, life tables of T. vaporariorium were established at daily fluctuating temperature in two different environments: Cusco (5 °C-35 °C) and La Molina, Lima (13.7 °C-24.9 °C), and used to adjust and validate the model. The insect only completed its life cycle at constant temperatures above 15 °C and below 32 °C although the cycle was completed at daily fluctuating temperatures between 5 °C and 35 °C. The overall model portrayed population development within the temperature range of 14° to 32 °C with a maximum finite rate of population increase (= 1.14) at 23 °C. However, the model revealed poor convergence with life tables established at fluctuating temperatures indicating an influential effect of temperature fluctuations on the whitefly life history parameters, particularly on adult survival time and reproduction. Therefore, we adjusted the model for convergence with a single life table observed at fluctuating temperature. The adjusted model gave good predictions when compared with remaining observed life tables and published data. The adjusted model predicted population development within the temperature range of 11.5 °C-35.5 °C, and maximum population growth at around 24 °C with a finite rate of increase, λ, of 1.137 and a population doubling time of 5 days. The established process-based physiological model presented here for T. vaporariorum can be used for predicting the species distribution potential based on temperature worldwide and should prove helpful in adjusting pest management measures. Moreover, the information obtained will be used to predict the spread potential of potato yellow vein disease.

Project description:The glasshouse whitefly (Trialeurodes vaporariorum Westwood) is a polyphagous arthropod pest that is of particular detriment to glasshouse grown tomato (Solanum lycopersicum) across temperate regions of the world. Control of whiteflies with synthetic pesticides has resulted in the evolution of resistant genotypes and a reduction in natural enemies, thus highlighting the need for environmentally sound control strategies. Volatile organic compounds (VOCs) offer an environmentally benign alternative to synthetic chemical sprays and this study explored the use of VOCs as insect repellents and plant defence elicitors to control whiteflies on tomato in a commercial glasshouse setting. Limonene in the form of a volatile dispenser system was found to successfully repel whitefly from the target crop and increased fruit yield by 32% during a heavy whitefly infestation. Analysis of tomato herbivore induced plant volatiles (HIPVs) led us to select methyl salicylate (MeSA) as the plant elicitor and application of MeSA to un-infested tomato plants was found to successfully reduce whitefly population development and increase yield by 11%, although this difference was marginally statistically significant. Combination of these two methods was also effective but whitefly abundance in combined plots was similar to the standalone limonene treatment across the course of the experiment. All of the VOC based control methods we used had a negative impact on whitefly performance, with more pronounced effects during the first few weeks of infestation. In subsequent laboratory experiments, we found elevated peroxidase (POD) activity and a significant increase in TPX1 and PR1 transcripts in MeSA treated plants. This led us to deduce that MeSA immediately induced plant defences, rather than priming them. We did however see evidence for residual priming, as plants treated with MeSA and infested with whiteflies produced significantly higher levels of POD activity than whitefly infestation alone. Despite the fact that our treatments failed to synergise, our methods can be optimised further, and the effectiveness of the standalone treatments is promising for future studies. In particular, our repellent limonene dispensers were extremely effective at deterring whiteflies and offer a low economic cost and easy to implement whitefly control option. The methods we have used here could be incorporated into current integrated pest management (IPM) systems, a sustainable approach to pest control which will be central to our efforts to manage whitefly populations under glass in the future.

Dataset Information

Volatile-Mediated Attraction of Greenhouse Whitefly Trialeurodes vaporariorum to Tomato and Eggplant.

Publications

Volatile-Mediated Attraction of Greenhouse Whitefly <i>Trialeurodes vaporariorum</i> to Tomato and Eggplant.

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