Ontology highlight
ABSTRACT:
SUBMITTER: June Byun S
PROVIDER: S-EPMC5517518 | biostudies-literature | 2017 Jul
REPOSITORIES: biostudies-literature
June Byun Sung S Yuk Seong-Su SS Jang Ye-Jin YJ Choi Hoonsung H Jeon Mi-Hyang MH Erdene-Ochir T O TO Kwon Jung-Hoon JH Noh Jin-Yong JY Sun Kim Jeom J Gyu Yoo Jae J Song Chang-Seon CS
Scientific reports 20170719 1
The 3D8 single chain variable fragment (scFv) is a mini-antibody that causes unusual sequence-independent nuclease activity against all types of nucleic acids. We used recombinant lentiviruses to generate transgenic chickens expressing the 3D8 scFv gene under the control of the chicken β-actin promoter. From 420 injected embryos, 200 chicks (G0) hatched and were screened for the 3D8 scFv using PCR, and 15 chicks were identified as transgenic birds expressing the transgene in their semen. The G0 ...[more]