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Endothelial Dysfunction in Severe Preeclampsia is Mediated by Soluble Factors, Rather than Extracellular Vesicles.

ABSTRACT: In severe early-onset preeclampsia (sPE) the placenta releases soluble angiogenesis-regulating proteins, trophoblast-derived fragments, and extracellular vesicles (EVs). Their relative importance in disease pathogenesis is not presently understood. We explanted placental villi from healthy and sPE women then separated the media into: total-conditioned, EV-depleted and EV-enriched media. Three fractions were compared for; angiogenic protein secretion by ELISA, angiogenic and inflammation gene mRNA expression and leukocyte adhesion assay. sPE placental villi secreted significantly less PlGF (70?±?18?pg/mL) than preterm controls (338?±?203; p?=?0.03). sFlt-1:PlGF ratios in total-conditioned (115?±?29) and EV-depleted media (136?±?40) from sPE placental villi were significantly higher than in EV-enriched media (42?±?12; p?

SUBMITTER: O'Brien M

PROVIDER: S-EPMC5517616 | biostudies-literature | 2017 Jul

REPOSITORIES: biostudies-literature

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Endothelial Dysfunction in Severe Preeclampsia is Mediated by Soluble Factors, Rather than Extracellular Vesicles.

O'Brien Michelle M Baczyk Dora D Kingdom John C JC

Scientific reports 20170719 1

In severe early-onset preeclampsia (sPE) the placenta releases soluble angiogenesis-regulating proteins, trophoblast-derived fragments, and extracellular vesicles (EVs). Their relative importance in disease pathogenesis is not presently understood. We explanted placental villi from healthy and sPE women then separated the media into: total-conditioned, EV-depleted and EV-enriched media. Three fractions were compared for; angiogenic protein secretion by ELISA, angiogenic and inflammation gene mRN ...[more]

PMID: 28725005

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