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Cerebrospinal fluid humoral immunity in the differential diagnosis of multiple sclerosis.

ABSTRACT:

Background

The diagnostic accuracy of cerebrospinal fluid oligoclonal bands (CSF-OCB) detected by isoelectric focusing (IEF) in patients with multiple sclerosis (MS) was evaluated in our study.

Methods

Three hundred and twenty-one patients with MS and other central nervous system (CNS) immune mediated disorders were assessed (CIMD). Cerebrospinal fluid and matched serum samples were examined for the presence of OCB by IEF-IB (isoelectric focusing with immunoblotting).

Results

Isolated oligoclonal bands (ISO-OCB) were the only predictor of MS diagnosis independent of age, gender and CSF-OCB. ISO-OCB ? 3.5 detected by IEF yielded a sensitivity of 98% and specificity of 87% in distinguishing MS from MS mimickers.

Conclusions

For the neurologist, a score of ? 4 ISO-OCB supports the diagnosis of MS. On the other hand, ISO-OCB ?3 favors CIMD. Further studies with larger population samples are warranted to confirm these findings.

SUBMITTER: Bernitsas E

PROVIDER: S-EPMC5519077 | biostudies-literature | 2017

REPOSITORIES: biostudies-literature

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Cerebrospinal fluid humoral immunity in the differential diagnosis of multiple sclerosis.

Bernitsas Evanthia E Khan Omar O Razmjou Sara S Tselis Alexandros A Bao Fen F Caon Christina C Millis Scott S Seraji-Bozorgzad Navid N

PloS one 20170720 7

<h4>Background</h4>The diagnostic accuracy of cerebrospinal fluid oligoclonal bands (CSF-OCB) detected by isoelectric focusing (IEF) in patients with multiple sclerosis (MS) was evaluated in our study.<h4>Methods</h4>Three hundred and twenty-one patients with MS and other central nervous system (CNS) immune mediated disorders were assessed (CIMD). Cerebrospinal fluid and matched serum samples were examined for the presence of OCB by IEF-IB (isoelectric focusing with immunoblotting).<h4>Results</ ...[more]

PMID: 28727770

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