Label-free three-dimensional imaging of Caenorhabditis elegans with visible optical coherence microscopy.

ABSTRACT: Fast, label-free, high-resolution, three-dimensional imaging platforms are crucial for high-throughput in vivo time-lapse studies of the anatomy of Caenorhabditis elegans, one of the most commonly used model organisms in biomedical research. Despite the needs, methods combining all these characteristics have been lacking. Here, we present label-free imaging of live Caenorhabditis elegans with three-dimensional sub-micrometer resolution using visible optical coherence microscopy (visOCM). visOCM is a versatile optical imaging method which we introduced recently for tomography of cell cultures and tissue samples. Our method is based on Fourier domain optical coherence tomography, an interferometric technique that provides three-dimensional images with high sensitivity, high acquisition rate

SUBMITTER: Coquoz S 

PROVIDER: S-EPMC5519216 | biostudies-literature | 2017

REPOSITORIES: biostudies-literature