Ontology highlight
ABSTRACT:
SUBMITTER: Beyersdorf MS
PROVIDER: S-EPMC5521581 | biostudies-literature | 2017 Aug
REPOSITORIES: biostudies-literature
Beyersdorf Matthew S MS Sircar Ria R Lookadoo Daniel B DB Bottone Cory J CJ Lynch Michael J MJ Crane Brian R BR Halkides Christopher J CJ
Protein science : a publication of the Protein Society 20170514 8
Phosphorylation of CheY promotes association with the flagellar motor and ultimately controls the directional bias of the motor. However, biochemical studies of activated CheY-phosphate have been challenging due to the rapid hydrolysis of the aspartyl-phosphate in vitro. An inert analog of Tm CheY-phosphate, phosphono-CheY, was synthesized by chemical modification and purified by cation-exchange chromatography. Changes in HPLC retention times, chemical assays for phosphate and free thiol, and ma ...[more]