Ontology highlight
ABSTRACT:
SUBMITTER: Kurihara T
PROVIDER: S-EPMC5522428 | biostudies-literature | 2017 Jul
REPOSITORIES: biostudies-literature
Kurihara Takeshi T Fukuhara Takasuke T Ono Chikako C Yamamoto Satomi S Uemura Kentaro K Okamoto Toru T Sugiyama Masaya M Motooka Daisuke D Nakamura Shota S Ikawa Masato M Mizokami Masashi M Maehara Yoshihiko Y Matsuura Yoshiharu Y
Scientific reports 20170721 1
Complete removal of hepatitis B virus (HBV) DNA from nuclei is difficult by the current therapies. Recent reports have shown that a novel genome-editing tool using Cas9 with a single-guide RNA (sgRNA) system can cleave the HBV genome in vitro and in vivo. However, induction of a double-strand break (DSB) on the targeted genome by Cas9 risks undesirable off-target cleavage on the host genome. Nickase-Cas9 cleaves a single strand of DNA, and thereby two sgRNAs are required for inducing DSBs. To av ...[more]