Unknown

Dataset Information

0

Cytoplasmic Dynein Transports Axonal Microtubules in a Polarity-Sorting Manner.


ABSTRACT: Axonal microtubules are predominantly organized into a plus-end-out pattern. Here, we tested both experimentally and with computational modeling whether a motor-based polarity-sorting mechanism can explain this microtubule pattern. The posited mechanism centers on cytoplasmic dynein transporting plus-end-out and minus-end-out microtubules into and out of the axon, respectively. When cytoplasmic dynein was acutely inhibited, the bi-directional transport of microtubules in the axon was disrupted in both directions, after which minus-end-out microtubules accumulated in the axon over time. Computational modeling revealed that dynein-mediated transport of microtubules can establish and preserve a predominantly plus-end-out microtubule pattern as per the details of the experimental findings, but only if a kinesin motor and a static cross-linker protein are also at play. Consistent with the predictions of the model, partial depletion of TRIM46, a protein that cross-links axonal microtubules in a manner that influences their polarity orientation, leads to an increase in microtubule transport.

SUBMITTER: Rao AN 

PROVIDER: S-EPMC5523108 | biostudies-literature | 2017 Jun

REPOSITORIES: biostudies-literature

altmetric image

Publications

Cytoplasmic Dynein Transports Axonal Microtubules in a Polarity-Sorting Manner.

Rao Anand N AN   Patil Ankita A   Black Mark M MM   Craig Erin M EM   Myers Kenneth A KA   Yeung Howard T HT   Baas Peter W PW  

Cell reports 20170601 11


Axonal microtubules are predominantly organized into a plus-end-out pattern. Here, we tested both experimentally and with computational modeling whether a motor-based polarity-sorting mechanism can explain this microtubule pattern. The posited mechanism centers on cytoplasmic dynein transporting plus-end-out and minus-end-out microtubules into and out of the axon, respectively. When cytoplasmic dynein was acutely inhibited, the bi-directional transport of microtubules in the axon was disrupted i  ...[more]

Similar Datasets

| S-EPMC4137274 | biostudies-literature
| S-EPMC4410804 | biostudies-other
| S-EPMC2409227 | biostudies-literature
| S-EPMC6124060 | biostudies-literature
| S-EPMC4579568 | biostudies-literature
| S-EPMC4443381 | biostudies-literature
| S-EPMC4706479 | biostudies-literature
| S-EPMC6719453 | biostudies-literature
| S-EPMC4947180 | biostudies-literature
| S-EPMC2604933 | biostudies-literature