In vivo assessment of intracellular redox state in rat liver using hyperpolarized [1-13 C]Alanine.

ABSTRACT: The intracellular lactate to pyruvate concentration ratio is a commonly used tissue assay biomarker of redox, being proportional to free cytosolic [NADH]/[NAD+ ]. In this study, we assessed the use of hyperpolarized [1-13 C]alanine and the subsequent detection of the intracellular products of [1-13 C]pyruvate and [1-13 C]lactate as a useful substrate for assessing redox levels in the liver in vivo.Animal experiments were conducted to measure in vivo metabolism at baseline and after ethanol infusion. A solution of 80-mM hyperpolarized [1-13 C]alanine was injected intravenously at baseline (n?=?8) and 45?min after ethanol infusion (n?=?4), immediately followed by the dynamic acquisition of 13 C MRS spectra.In vivo rat liver spectra showed peaks from [1-13 C] alanine and the products of [1-13 C]lactate, [1-13 C]pyruvate, and 13 C-bicarbonate. A significantly increased 13 C-lactate/13 C-pyruvate ratio was observed after ethanol infusion (8.46?±?0.58 at baseline versus 13.58?±?0.69 after ethanol infusion; P?

SUBMITTER: Park JM 

PROVIDER: S-EPMC5523144 | biostudies-literature | 2017 May

REPOSITORIES: biostudies-literature