Fitness of Antibiotic-Resistant Bacteria in the Environment: A Laboratory Activity.

ABSTRACT: In this laboratory experiment, we propose an opportunity for students to broaden their understanding of the ecology of antibiotic-resistant and sensitive waterborne bacteria. Antibiotics can be found in rivers or soil as a consequence of agricultural practices or as a result of human use. Concentrations of antibiotics in the environment may range from a few ng to μg L-1. Such concentrations can affect the selection and fitness of resistant bacteria. In this laboratory activity, students learn how to set up a fitness experiment by using an isogenic pair of antibiotic-resistant and sensitive bacteria in the presence or absence of selective pressure. Microcosms were generated by using filtered river water containing populations of resistant and sensitive bacteria. Competition of both populati

SUBMITTER: Marvasi M 

PROVIDER: S-EPMC5524443 | biostudies-literature | 2017

REPOSITORIES: biostudies-literature