The Tnfaip8-PE complex is a novel upstream effector in the anti-autophagic action of insulin.

ABSTRACT: Defective hepatic autophagy is observed in obesity and diabetes, whereas autophagy is inhibited by insulin in hepatocytes. Insulin-induced anti-autophagy is mediated by non-canonical G?i3 signaling via an unknown mechanism. Previously, we identified the anti-autophagic activity of Tnfaip8 via activation of mammalian target of rapamycin (mTOR) in the nervous system. Here, we demonstrate that insulin temporally induces Tnfaip8, which mediates the anti-autophagic action of insulin through formation of a novel ternary complex including Tnfaip8, phosphatidylethanolamine (PE) and G?i3. Specifically, an X-ray crystallographic study of Tnfaip8 from Mus musculus (mTnfaip8) at 2.03?Å together with LC-MS analyses reveals PE in the hydrophobic cavity. However, an mTnfaip8 mutant lacking PE does not interact with G?i3, indicating that the PE component is critical for the anti-autophagic action of mTnfaip8 via interaction with G?i3. Therefore, the mTnfaip8-PE complex may act as an essential upstream effector via ternary complex formation most likely with active G?i3 during insulin-induced anti-autophagy.

SUBMITTER: Kim JS 

PROVIDER: S-EPMC5524748 | biostudies-literature | 2017 Jul

REPOSITORIES: biostudies-literature