Mesenchymal Stromal Cells Accelerate Epithelial Tight Junction Assembly via the AMP-Activated Protein Kinase Pathway, Independently of Liver Kinase B1.

ABSTRACT:

Background

Mesenchymal stromal cells (MSC) are fibroblast-like multipotent cells capable of tissue-repair properties. Given the essentiality of tight junctions (TJ) in epithelial integrity, we hypothesized that MSC modulate TJ formation, via the AMP-activated kinase (AMPK) pathway. Liver kinase-β1 (LKB1) and Ca²⁺-calmodulin-dependent protein kinase kinase (CaMKK) represent the main kinases that activate AMPK.

Methods

The in vitro Ca²⁺ switch from 5 μM to 1.8 mM was performed using epithelial Madin-Darby canine kidney (MDCK) cells cultured alone or cocultured with rat bone marrow-derived MSC or preexposed to MSC-conditioned medium. TJ assembly was measured by assessing ZO-1 relocation to cell-cell contacts. Experiments were conducted using MDCK stably expressing short-hairpin-RNA (shRNA) against LKB1 or luciferase (LUC, as controls). Compound STO-609 (50 μM) was used as CaMKK inhibitor.

Results

Following Ca²⁺ switch, ZO-1 relocation and phosphorylation/activation of AMPK were significantly higher in MDCK/MSC compared to MDCK. No difference in AMPK phosphorylation was observed between LKB1-shRNA and Luc-shRNA MDCK following Ca²⁺ switch. Conversely, incubation with STO-609 prior to Ca²⁺ switch prevented AMPK phosphorylation and ZO-1 relocation. MSC-conditioned medium slightly but significantly increased AMPK activation and accelerated TJ-associated distribution of ZO-1 post Ca²⁺ switch in comparison to regular medium.

Conclusions

MSC modulate the assembly of epithelial TJ, via the CaMKK/AMPK pathway independently of LKB1.

SUBMITTER: Rowart P 

PROVIDER: S-EPMC5525096 | biostudies-literature | 2017

REPOSITORIES: biostudies-literature