Dataset Information

Comparison of Three PCR-based Methods for Simplicity and Cost Effectiveness Identification of Cutaneous Leishmaniasis Due to Leishmania tropica.

ABSTRACT:

Background

To compare three molecular methods, PCR-RFLP for internal transcribed spacer, PCR sequencing and high resolution melting analysis shown reliable sensitivity and specificity for detecting Leishmania tropica as a model for cutaneous leishmaniasis (CL) as the perspective overview for scientific and economic approaches.

Methods

This study was carried out between 2015 and 2016 in Leishmaniasis Research Center in Kerman University of Medical Sciences, Kerman, Iran. The positives smears (n=50) were obtained from patients referred from the health clinics in a major anthroponotic CL (ACL) focus, southeastern Iran. Only smear preparations with the same grade were selected according to the method described by the WHO for future PCR assays.

Results

All three molecular methods had capability to identify positive samples at species level with the same specificity and sensitivity. However, these techniques were different in simplicity, consuming time, and cost effectiveness. Although additional enzymatic process in PCR-RFLP provided good resolution to find Leishmania species but this would cause time and cost increases.

Conclusion

HRM (high resolution melting) is a relatively new technique that allows direct characterization of PCR amplicons in a closed system with more simplicity, cost effectiveness and time-consuming compared with other PCR-based assays for epidemiological or clinical identification purposes.

SUBMITTER: Mohammadi MA

PROVIDER: S-EPMC5527031 | biostudies-literature | 2017 Apr-Jun

REPOSITORIES: biostudies-literature

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Publications

Comparison of Three PCR-based Methods for Simplicity and Cost Effectiveness Identification of Cutaneous Leishmaniasis Due to <i>Leishmania tropica</i>.

Mohammadi Mohammad Ali MA Bamorovat Mehdi M Fasihi Harandi Majid M Karimi Tayyebeh T Sharifi Iraj I Aflatoonian Mohammad Reza MR

Iranian journal of parasitology 20170401 2

<h4>Background</h4>To compare three molecular methods, PCR-RFLP for internal transcribed spacer, PCR sequencing and high resolution melting analysis shown reliable sensitivity and specificity for detecting <i>Leishmania tropica</i> as a model for cutaneous leishmaniasis (CL) as the perspective overview for scientific and economic approaches.<h4>Methods</h4>This study was carried out between 2015 and 2016 in Leishmaniasis Research Center in Kerman University of Medical Sciences, Kerman, Iran. The ...[more]

PMID: 28761481

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