Quantitative analysis of protein interaction network dynamics in yeast.

ABSTRACT: Many cellular functions are mediated by protein-protein interaction networks, which are environment dependent. However, systematic measurement of interactions in diverse environments is required to better understand the relative importance of different mechanisms underlying network dynamics. To investigate environment-dependent protein complex dynamics, we used a DNA-barcode-based multiplexed protein interaction assay in Saccharomyces cerevisiae to measure in vivo abundance of 1,379 binary protein complexes under 14 environments. Many binary complexes (55%) were environment dependent, especially those involving transmembrane transporters. We observed many concerted changes around highly connected proteins, and overall network dynamics suggested that "concerted" protein-center

SUBMITTER: Celaj A 

PROVIDER: S-EPMC5527849 | biostudies-literature | 2017 Jul

REPOSITORIES: biostudies-literature