Biophysical characterization of the calmodulin-like domain of Plasmodium falciparum calcium dependent protein kinase 3.

ABSTRACT: Calcium dependent protein kinases are unique to plants and certain parasites and comprise an N-terminal segment and a kinase domain that is regulated by a C-terminal calcium binding domain. Since the proteins are not found in man they are potential drug targets. We have characterized the calcium binding lobes of the regulatory domain of calcium dependent protein kinase 3 from the malaria parasite Plasmodium falciparum. Despite being structurally similar, the two lobes differ in several other regards. While the monomeric N-terminal lobe changes its structure in response to calcium binding and shows global dynamics on the sub-millisecond time-scale both in its apo and calcium bound states, the C-terminal lobe could not be prepared calcium-free and forms dimers in solution. If our results can

SUBMITTER: Andresen C 

PROVIDER: S-EPMC5528832 | biostudies-literature | 2017

REPOSITORIES: biostudies-literature