Ontology highlight
ABSTRACT:
SUBMITTER: Cobellis G
PROVIDER: S-EPMC552971 | biostudies-literature | 2005 Mar
REPOSITORIES: biostudies-literature
Cobellis Gilda G Nicolaus Giancarlo G Iovino Mariangela M Romito Antonio A Marra Emanuele E Barbarisi Manlio M Sardiello Marco M Di Giorgio Francesco P FP Iovino Nicola N Zollo Massimo M Ballabio Andrea A Cortese Riccardo R
Nucleic acids research 20050301 4
In an effort to make transgenesis more flexible and reproducible, we developed a system based on novel 5' and 3' 'gene trap' vectors containing heterospecific Flp recognition target sites and the corresponding 'exchange' vectors allowing the insertion of any DNA sequence of interest into the trapped locus. Flp-recombinase-mediated cassette exchange was demonstrated to be highly efficient in our system, even in the absence of locus-specific selection. The feasibility of constructing a library of ...[more]