Ontology highlight
ABSTRACT:
SUBMITTER: Prevedel R
PROVIDER: S-EPMC5531274 | biostudies-literature | 2016 Dec
REPOSITORIES: biostudies-literature
Nature methods 20161031 12
Although whole-organism calcium imaging in small and semi-transparent animals has been demonstrated, capturing the functional dynamics of large-scale neuronal circuits in awake behaving mammals at high speed and resolution has remained one of the main frontiers in systems neuroscience. Here we present a method based on light sculpting that enables unbiased single- and dual-plane high-speed (up to 160 Hz) calcium imaging as well as in vivo volumetric calcium imaging of a mouse cortical column (0. ...[more]