DNA Microarray Analysis of Estrogen Responsive Genes in Ishikawa Cells by Glabridin.

ABSTRACT: Based on a previous study, glabridin displayed a dose-dependent increase in estrogenic activity and cell proliferative activity in Ishikawa cells. However, when treated in combination with 17?-E2, synergistic estrogenic effect was observed but without the same synergistic increase in cell proliferative effect. This study aimed to identify the estrogen and nonestrogen-regulated activities induced by glabridin and in combination with 17?-E2 in comparison with 17?-E2. The results showed that 10?µM glabridin and the combination treatment of 100?nM glabridin with 1?nM 17?-E2 regulated both the genomic and nongenomic estrogen pathways to possibly provide benefits of estrogens in cardiovascular, circulatory, and vasculature systems. Meanwhile, the combination of 100?nM glabridin with 1?nM 17?-E2 seems to be more suitable to be used as an estrogen replacement. Finally, the results of this study have added on to the present knowledge of glabridin's function as a phytoestrogen and suggested new ideas for the usage of glabridin.

SUBMITTER: Melissa PSW 

PROVIDER: S-EPMC5533267 | biostudies-literature | 2017

REPOSITORIES: biostudies-literature