Unknown

Dataset Information

0

Microfluidic-based high-throughput optical trapping of nanoparticles.


ABSTRACT: Optical tweezers have emerged as a powerful tool for multiparametric analysis of individual nanoparticles with single-molecule sensitivity. However, its inherent low-throughput characteristic remains a major obstacle to its applications within and beyond the laboratory. This limitation is further exacerbated when working with low concentration nanoparticle samples. Here, we present a microfluidic-based optical tweezers system that can 'actively' deliver nanoparticles to a designated microfluidic region for optical trapping and analysis. The active microfluidic delivery of nanoparticles results in significantly improved throughput and efficiency for optical trapping of nanoparticles. We observed a more than tenfold increase in optical trapping throughput for nanoparticles as compared to conventional systems at the same nanoparticle concentration. To demonstrate the utility of this microfluidic-based optical tweezers system, we further used back-focal plane interferometry coupled with a trapping laser for the precise quantitation of nanoparticle size without prior knowledge of the refractive index of nanoparticles. The development of this microfluidic-based active optical tweezers system thus opens the door to high-throughput multiparametric analysis of nanoparticles using precision optical traps in the future.

SUBMITTER: Kotnala A 

PROVIDER: S-EPMC5533511 | biostudies-literature | 2017 Jun

REPOSITORIES: biostudies-literature

altmetric image

Publications

Microfluidic-based high-throughput optical trapping of nanoparticles.

Kotnala Abhay A   Zheng Yi Y   Fu Jianping J   Cheng Wei W  

Lab on a chip 20170601 12


Optical tweezers have emerged as a powerful tool for multiparametric analysis of individual nanoparticles with single-molecule sensitivity. However, its inherent low-throughput characteristic remains a major obstacle to its applications within and beyond the laboratory. This limitation is further exacerbated when working with low concentration nanoparticle samples. Here, we present a microfluidic-based optical tweezers system that can 'actively' deliver nanoparticles to a designated microfluidic  ...[more]

Similar Datasets

| S-EPMC8000121 | biostudies-literature
| S-EPMC6644722 | biostudies-literature
| S-EPMC8166820 | biostudies-literature
| S-EPMC7328786 | biostudies-literature
2024-08-27 | GSE255889 | GEO
| S-EPMC10197168 | biostudies-literature
| S-EPMC10502464 | biostudies-literature
| S-EPMC3161570 | biostudies-literature
| S-EPMC4073585 | biostudies-literature
| S-EPMC8366422 | biostudies-literature