Unknown

Dataset Information

0

LAMP assay for rapid diagnosis of cow DNA in goat milk and meat samples.


ABSTRACT: Animal species detection is one of the crucial steps for consumer's food analysis. In the present study we developed an in-house built loop-mediated isothermal amplification (LAMP) assay for rapid detection of adulterated cow DNA in goat milk/meat samples. The cow milk/tissue DNA in goat milk/meat samples were identified in the developed LAMP assay by either naked eye visualizing with SYBR Green I dyes or by detecting the typical ladder pattern on gel electrophoresis. This test can detect up to minimum 5% level of cow components admixed in goat milk/meat samples and can be completed within 1 h 40 min starting from DNA extraction from milk/meat samples and can be performed in a water bath. Developed LAMP methodology is simple; rapid and sensitive techniques that can detect adulterant like cow components in goat milk/meat are more accurate than other existing DNA based technologies.

SUBMITTER: Deb R 

PROVIDER: S-EPMC5534258 | biostudies-literature | 2017

REPOSITORIES: biostudies-literature

altmetric image

Publications

LAMP assay for rapid diagnosis of cow DNA in goat milk and meat samples.

Deb R R   Sengar G S GS   Singh U U   Kumar S S   Raja T V TV   Alex R R   Alyethodi R R RR   Prakash B B  

Iranian journal of veterinary research 20170101 2


Animal species detection is one of the crucial steps for consumer's food analysis. In the present study we developed an in-house built loop-mediated isothermal amplification (LAMP) assay for rapid detection of adulterated cow DNA in goat milk/meat samples. The cow milk/tissue DNA in goat milk/meat samples were identified in the developed LAMP assay by either naked eye visualizing with SYBR Green I dyes or by detecting the typical ladder pattern on gel electrophoresis. This test can detect up to  ...[more]

Similar Datasets

2023-05-10 | GSE227559 | GEO
| S-EPMC6835441 | biostudies-literature
| PRJNA782675 | ENA
| S-EPMC4071038 | biostudies-literature
| S-EPMC11302832 | biostudies-literature
| S-EPMC5491318 | biostudies-literature
| S-EPMC7552020 | biostudies-literature
| S-EPMC4945066 | biostudies-literature
| S-EPMC10094125 | biostudies-literature
| S-EPMC8415568 | biostudies-literature