Unknown

Dataset Information

0

The Chemokine Receptor CXCR6 Evokes Reverse Signaling via the Transmembrane Chemokine CXCL16.


ABSTRACT: Reverse signaling is a signaling mechanism where transmembrane or membrane-bound ligands transduce signals and exert biological effects upon binding of their specific receptors, enabling a bidirectional signaling between ligand and receptor-expressing cells. In this study, we address the question of whether the transmembrane chemokine (C-X-C motif) ligand 16, CXCL16 is able to transduce reverse signaling and investigate the biological consequences. For this, we used human glioblastoma cell lines and a melanoma cell line as in vitro models to show that stimulation with recombinant C-X-C chemokine receptor 6 (CXCR6) or CXCR6-containing membrane preparations induces intracellular (reverse) signaling. Specificity was verified by RNAi experiments and by transfection with expression vectors for the intact CXCL16 and an intracellularly-truncated form of CXCL16. We showed that reverse signaling via CXCL16 promotes migration in CXCL16-expressing melanoma and glioblastoma cells, but does not affect proliferation or protection from chemically-induced apoptosis. Additionally, fast migrating cells isolated from freshly surgically-resected gliomas show a differential expression pattern for CXCL16 in comparison to slowly-migrating cells, enabling a possible functional role of the reverse signaling of the CXCL16/CXCR6 pair in human brain tumor progression in vivo.

SUBMITTER: Adamski V 

PROVIDER: S-EPMC5535959 | biostudies-literature |

REPOSITORIES: biostudies-literature

Similar Datasets

| S-EPMC4546458 | biostudies-literature
| S-EPMC8326113 | biostudies-literature
| S-EPMC8306453 | biostudies-literature
| S-EPMC6653424 | biostudies-literature
| S-EPMC8071165 | biostudies-literature
| S-EPMC6479025 | biostudies-literature
| S-EPMC7748218 | biostudies-literature
| S-EPMC5340378 | biostudies-literature
| S-EPMC4814243 | biostudies-literature
| S-EPMC2740747 | biostudies-literature