Ontology highlight
ABSTRACT:
SUBMITTER: Fox JM
PROVIDER: S-EPMC5536152 | biostudies-literature | 2017 Aug
REPOSITORIES: biostudies-literature
Fox James M JM Wiggins Rebecca C RC Moore John W J JWJ Brewer Christine C Hunter Bill B Andrew Alison C AC Martin Fabiola F
Cryobiology 20170609
<h4>Background</h4>In order to conduct laboratory studies on donated cervical tissue at suitable times an effective and reliable cryopreservation protocol for cervical tissue is required.<h4>Methods</h4>An active freezing approach was devised utilising 10% dimethyl sulfoxide in foetal bovine serum as a cryoprotective agent with a cooling rate of 1 °C/min to -50 °C then 10 °C/min to -120 °C; a related thawing protocol was also optimised which would allow for the bio-banking of cervical tissue. Vi ...[more]