Ontology highlight
ABSTRACT:
SUBMITTER: Miyashita K
PROVIDER: S-EPMC5538281 | biostudies-literature | 2017 Aug
REPOSITORIES: biostudies-literature
Miyashita Kazuya K Nakajima Katsuyuki K Fukamachi Isamu I Muraba Yuji Y Koga Takafumi T Shimomura Yohnosuke Y Machida Tetsuyo T Murakami Masami M Kobayashi Junji J
Journal of lipid research 20170620 8
There is no established method for measuring human hepatic triglyceride (TG) lipase (HTGL) concentration in serum. In this study, we developed new monoclonal Abs (MoAbs) (9A1 mouse MoAb and 141A1 rat MoAb) that react with HTGL both in serum and in postheparin plasma (PHP) and established a novel ELISA system for measuring serum HTGL and PHP-HTGL concentrations. To confirm the specificity of MoAbs, we performed immunoprecipitation-immunoblotting analysis. Both 9A1 mouse MoAb and 141A1 rat MoAb we ...[more]