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DNA barcoding of odonates from the Upper Plata basin: Database creation and genetic diversity estimation.


ABSTRACT: We present a DNA barcoding study of Neotropical odonates from the Upper Plata basin, Brazil. A total of 38 species were collected in a transition region of "Cerrado" and Atlantic Forest, both regarded as biological hotspots, and 130 cytochrome c oxidase subunit I (COI) barcodes were generated for the collected specimens. The distinct gap between intraspecific (0-2%) and interspecific variation (15% and above) in COI, and resulting separation of Barcode Index Numbers (BIN), allowed for successful identification of specimens in 94% of cases. The 6% fail rate was due to a shared BIN between two separate nominal species. DNA barcoding, based on COI, thus seems to be a reliable and efficient tool for identifying Neotropical odonate specimens down to the species level. These results underscore the utility of DNA barcoding to aid specimen identification in diverse biological hotspots, areas that require urgent action regarding taxonomic surveys and biodiversity conservation.

SUBMITTER: Koroiva R 

PROVIDER: S-EPMC5538745 | biostudies-literature | 2017

REPOSITORIES: biostudies-literature

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DNA barcoding of odonates from the Upper Plata basin: Database creation and genetic diversity estimation.

Koroiva Ricardo R   Pepinelli Mateus M   Rodrigues Marciel Elio ME   Roque Fabio de Oliveira FO   Lorenz-Lemke Aline Pedroso AP   Kvist Sebastian S  

PloS one 20170801 8


We present a DNA barcoding study of Neotropical odonates from the Upper Plata basin, Brazil. A total of 38 species were collected in a transition region of "Cerrado" and Atlantic Forest, both regarded as biological hotspots, and 130 cytochrome c oxidase subunit I (COI) barcodes were generated for the collected specimens. The distinct gap between intraspecific (0-2%) and interspecific variation (15% and above) in COI, and resulting separation of Barcode Index Numbers (BIN), allowed for successful  ...[more]

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