Adenosine Triphosphate-Encapsulated Liposomes with Plasmonic Nanoparticles for Surface Enhanced Raman Scattering-Based Immunoassays.

ABSTRACT: In this study, we prepared adenosine triphosphate (ATP) encapsulated liposomes, and assessed their applicability for the surface enhanced Raman scattering (SERS)-based assays with gold-silver alloy (Au@Ag)-assembled silica nanoparticles (NPs; SiO?@Au@Ag). The liposomes were prepared by the thin film hydration method from a mixture of l-?-phosphatidylcholine, cholesterol, and PE-PEG2000 in chloroform; evaporating the solvent, followed by hydration of the resulting thin film with ATP in phosphate-buffered saline (PBS). Upon lysis of the liposome, the SERS intensity of the SiO?@Au@Ag NPs increased with the logarithm of number of ATP-encapsulated liposomes after lysis in the range of 8 × 10? to 8 × 10¹⁰. The detection limit of liposome was calculated to be 1.3 × 10^-17 mol. The successful application of ATP-encapsulated liposomes to SiO?@Au@Ag NPs based SERS analysis has opened a new avenue for Raman label chemical (RCL)-encapsulated liposome-enhanced SERS-based immunoassays.

SUBMITTER: Pham XH 

PROVIDER: S-EPMC5539552 | biostudies-literature | 2017 Jun

REPOSITORIES: biostudies-literature