Inhibition of Rho Kinase Induces Antioxidative Molecules and Suppresses Reactive Oxidative Species in Trabecular Meshwork Cells.

ABSTRACT:

Purpose

To investigate the effect of rho kinase inhibitors on oxidative stress in trabecular meshwork (TM) cells.

Methods

TM cells were isolated from the eyes of cynomolgus monkeys. Y-27632 and menadione were used to inhibit rho kinase and induce production of reactive oxygen species (ROS), respectively. The cynomolgus monkey array and 12,613 probes were used in DNA microarray analysis, and the affected genes were categorized using gene ontology analysis. The mRNA levels of the target genes were confirmed by real-time RT-PCR. Intracellular oxidative stress was detected using a fluorescent reagent sensitive to ROS. Cell viability was assessed by the WST-8 assay.

Results

Gene ontology analysis revealed upregulation of genes involved in antioxidant activity, and upregula

SUBMITTER: Fujimoto T 

PROVIDER: S-EPMC5540245 | biostudies-literature | 2017

REPOSITORIES: biostudies-literature